Exploring Preprocessed fMRI Data from fMRIPREP

Last updated on 2022-11-15 | Edit this page

Exploring Preprocessed fMRI Data from fMRIPREP

Overview

Questions

How does fMRIPrep store preprocessed neuroimaging data
How do I access preprocessed neuroimaging data

Objectives

Learn about fMRIPrep derivatives
Understand how preprocessed data is stored and how you can access key files for analysis

BIDS applications such as fMRIPREP output data into a full data structure with strong similarity to BIDS organization principals. In fact, there is a specification for derivatives (outputs derived from) BIDS datasets; although this is a current work in progress, details can be found in: BIDS Derivatives.

In this tutorial, we’ll explore the outputs generated by fMRIPREP and get a handle of how the data is organized from this preprocessing pipeline

Luckily the semi-standardized output for fMRIPrep is organized in such a way that the data is easily accessible using pyBIDS! We’ll first show what the full data structure looks like, then we will provide you with methods on how you can pull specific types of outputs using pyBIDS.

The fMRIPrep Derivative Data Structure

First let’s take a quick look at the fMRIPrep data structure:

BASH

tree -L 1 '../data/ds000030/derivatives/fmriprep'

OUTPUT


../data/ds000030/derivatives/fmriprep/
├── sub-10171
├── sub-10292
├── sub-10365
├── sub-10438
├── sub-10565
├── sub-10788
├── sub-11106
├── sub-11108
├── sub-11122
├── sub-11131
├── sub-50010
├── sub-50035
├── sub-50047
├── sub-50048
├── sub-50052
├── sub-50067
├── sub-50075
├── sub-50077
├── sub-50081
└── sub-50083

First note that inside the fMRIPrep folder, we have a folder per-subject. Let’s take a quick look at a single subject folder:

BASH

tree '../data/ds000030/derivatives/fmriprep/sub-10788'

OUTPUT

../data/ds000030/derivatives/fmriprep/sub-10788/
├── anat
│   ├── sub-10788_desc-aparcaseg_dseg.nii.gz
│   ├── sub-10788_desc-aseg_dseg.nii.gz
│   ├── sub-10788_desc-brain_mask.json
│   ├── sub-10788_desc-brain_mask.nii.gz
│   ├── sub-10788_desc-preproc_T1w.json
│   ├── sub-10788_desc-preproc_T1w.nii.gz
│   ├── sub-10788_dseg.nii.gz
│   ├── sub-10788_from-fsnative_to-T1w_mode-image_xfm.txt
│   ├── sub-10788_from-T1w_to-fsnative_mode-image_xfm.txt
│   ├── sub-10788_label-CSF_probseg.nii.gz
│   ├── sub-10788_label-GM_probseg.nii.gz
│   ├── sub-10788_label-WM_probseg.nii.gz
│   ├── sub-10788_space-MNI152NLin2009cAsym_desc-brain_mask.json
│   ├── sub-10788_space-MNI152NLin2009cAsym_desc-brain_mask.nii.gz
│   ├── sub-10788_space-MNI152NLin2009cAsym_desc-preproc_T1w.json
│   ├── sub-10788_space-MNI152NLin2009cAsym_desc-preproc_T1w.nii.gz
│   ├── sub-10788_space-MNI152NLin2009cAsym_dseg.nii.gz
│   ├── sub-10788_space-MNI152NLin2009cAsym_label-CSF_probseg.nii.gz
│   ├── sub-10788_space-MNI152NLin2009cAsym_label-GM_probseg.nii.gz
│   └── sub-10788_space-MNI152NLin2009cAsym_label-WM_probseg.nii.gz
├── func
│   ├── sub-10788_task-rest_desc-confounds_timeseries.json
│   ├── sub-10788_task-rest_desc-confounds_timeseries.tsv
│   ├── sub-10788_task-rest_from-scanner_to-T1w_mode-image_xfm.txt
│   ├── sub-10788_task-rest_from-T1w_to-scanner_mode-image_xfm.txt
│   ├── sub-10788_task-rest_space-MNI152NLin2009cAsym_boldref.nii.gz
│   ├── sub-10788_task-rest_space-MNI152NLin2009cAsym_desc-aparcaseg_dseg.nii.gz
│   ├── sub-10788_task-rest_space-MNI152NLin2009cAsym_desc-aseg_dseg.nii.gz
│   ├── sub-10788_task-rest_space-MNI152NLin2009cAsym_desc-brain_mask.json
│   ├── sub-10788_task-rest_space-MNI152NLin2009cAsym_desc-brain_mask.nii.gz
│   ├── sub-10788_task-rest_space-MNI152NLin2009cAsym_desc-preproc_bold.json
│   ├── sub-10788_task-rest_space-MNI152NLin2009cAsym_desc-preproc_bold.nii.gz
│   ├── sub-10788_task-rest_space-T1w_boldref.nii.gz
│   ├── sub-10788_task-rest_space-T1w_desc-aparcaseg_dseg.nii.gz
│   ├── sub-10788_task-rest_space-T1w_desc-aseg_dseg.nii.gz
│   ├── sub-10788_task-rest_space-T1w_desc-brain_mask.json
│   ├── sub-10788_task-rest_space-T1w_desc-brain_mask.nii.gz
│   ├── sub-10788_task-rest_space-T1w_desc-preproc_bold.json
│   └── sub-10788_task-rest_space-T1w_desc-preproc_bold.nii.gz
...

As you can see above, each subject folder is organized into an anat and func sub-folder.

Specifically:

the anat folder contains the preprocessed anatomical data. If multiple T1 files are available (all T1s even across sessions), then these data are merged - you will always have one anat folder under the subject folder
the func folder contains the preprocessed functional data. All tasks are dumped into the same folder and like the BIDS convention are indicated by the use of their filenames (task-[task_here])

Callout

This data is single-session, so a session folder is missing here - but with multiple sessions you will see anat and ses-[insert_session_here] folders where each session folder contain a func folder.

Hopefully you’re now convinced that the outputs of fMRIPREP roughly follows BIDS organization principles. The filenames themselves give you a full description of what each file is (check the slides to get an idea of what each file means!

Now let’s see how we can pull data in using pyBIDS!

Let’s import pyBIDS through the bids module first:

PYTHON

import bids

We can make a bids.BIDSLayout object as usual by just feeding in the fmriprep directory! However, there is one caveat… note that fMRIPrep doesn’t exactly adhere to the standard BIDS convention. It uses fields such as desc- which are not part of the original BIDS specification. I.e:

BASH

sub-10788_desc-preproc_T1w.nii.gz

In fact, BIDS allows for extensions which enable you to add additional fields to the standard BIDS convention (such as desc-!). fMRIprep uses the derivatives extension of the BIDS standard. pyBIDS can handle standard extensions to the BIDS specification quite easily:

PYTHON

layout = bids.BIDSLayout('../data/ds000030/derivatives/fmriprep/', config=['bids','derivatives'])

Now that we have a layout object, we can pretend like we’re working with a BIDS dataset! Let’s try some common commands that you would’ve used with a BIDS dataset:

First, we’ll demonstrate that we can grab a list of pre-processed subjects much like in the way we would grab subjects from a raw BIDS dataset:

PYTHON

layout.get_subjects()

OUTPUT

['10171',
 '10292',
 '10365',
 '10438',
 '10565',
 '10788',
 '11106',
 '11108',
 '11122',
 '11131',
 '50010',
 '50035',
 '50047',
 '50048',
 '50052',
 '50067',
 '50075',
 '50077',
 '50081',
 '50083']

We can also do the same for tasks

PYTHON

layout.get_tasks()

OUTPUT

['rest']

Now let’s try fetching specific files. Similar to how you would fetch BIDS data using pyBIDS, the exact same syntax will work for fMRIPREP derivatives. Let’s try pulling just the preprocessed anatomical data.

Recall that the anatomical folder is organized as follows:

BASH

tree '../data/ds000030/derivatives/fmriprep/sub-10788/anat'^

OUTPUT

../data/ds000030/derivatives/fmriprep/sub-10788/anat
├── sub-10788_desc-aparcaseg_dseg.nii.gz
├── sub-10788_desc-aseg_dseg.nii.gz
├── sub-10788_desc-brain_mask.json
├── sub-10788_desc-brain_mask.nii.gz
├── sub-10788_desc-preproc_T1w.json
├── sub-10788_desc-preproc_T1w.nii.gz
├── sub-10788_dseg.nii.gz
├── sub-10788_from-fsnative_to-T1w_mode-image_xfm.txt
├── sub-10788_from-T1w_to-fsnative_mode-image_xfm.txt
├── sub-10788_label-CSF_probseg.nii.gz
├── sub-10788_label-GM_probseg.nii.gz
├── sub-10788_label-WM_probseg.nii.gz
├── sub-10788_space-MNI152NLin2009cAsym_desc-brain_mask.json
├── sub-10788_space-MNI152NLin2009cAsym_desc-brain_mask.nii.gz
├── sub-10788_space-MNI152NLin2009cAsym_desc-preproc_T1w.json
├── sub-10788_space-MNI152NLin2009cAsym_desc-preproc_T1w.nii.gz
├── sub-10788_space-MNI152NLin2009cAsym_dseg.nii.gz
├── sub-10788_space-MNI152NLin2009cAsym_label-CSF_probseg.nii.gz
├── sub-10788_space-MNI152NLin2009cAsym_label-GM_probseg.nii.gz
└── sub-10788_space-MNI152NLin2009cAsym_label-WM_probseg.nii.gz

0 directories, 20 files

The file that we’re interested in is of form sub-[subject]_desc-preproc_T1w.nii.gz. Now we can construct a pyBIDS call to pull these types of files specifically:

PYTHON

preproc_T1 = layout.get(datatype='anat', desc='preproc', extension=".nii.gz")
preproc_T1

OUTPUT

[<BIDSImageFile filename='/home/jerry/projects/workshops/SDC-BIDS-fMRI/data/ds000030/derivatives/fmriprep/sub-10438/anat/sub-10438_desc-preproc_T1w.nii.gz'>,
 <BIDSImageFile filename='/home/jerry/projects/workshops/SDC-BIDS-fMRI/data/ds000030/derivatives/fmriprep/sub-10438/anat/sub-10438_space-MNI152NLin2009cAsym_desc-preproc_T1w.nii.gz'>,
 <BIDSImageFile filename='/home/jerry/projects/workshops/SDC-BIDS-fMRI/data/ds000030/derivatives/fmriprep/sub-10788/anat/sub-10788_desc-preproc_T1w.nii.gz'>,
 <BIDSImageFile filename='/home/jerry/projects/workshops/SDC-BIDS-fMRI/data/ds000030/derivatives/fmriprep/sub-10788/anat/sub-10788_space-MNI152NLin2009cAsym_desc-preproc_T1w.nii.gz'>,

Callout

If we didn’t configure pyBIDS with config=[‘bids’,‘derivatives’] then the desc keyword would not work!

Note that we also pulled in MNI152NLin2009cAsym_preproc.nii.gz data as well. This is data that has been transformed into MNI152NLin2009cAsym template space. We can pull this data out by further specifying our layout.get using the space argument:

PYTHON

mni_preproc_T1 = layout.get(datatype='anat',desc='preproc',extension='.nii.gz',space='MNI152NLin2009cAsym')
mni_preproc_T1

OUTPUT

[<BIDSImageFile filename='/home/jerry/projects/workshops/SDC-BIDS-fMRI/data/ds000030/derivatives/fmriprep/sub-10438/anat/sub-10438_space-MNI152NLin2009cAsym_desc-preproc_T1w.nii.gz'>,
 <BIDSImageFile filename='/home/jerry/projects/workshops/SDC-BIDS-fMRI/data/ds000030/derivatives/fmriprep/sub-10788/anat/sub-10788_space-MNI152NLin2009cAsym_desc-preproc_T1w.nii.gz'>,
 ...

What if we wanted to pull out the data in T1 “native space” (it really is a template space, since it is merged T1s)? Unfortunately for this isn’t directly possible using layout.get. Instead we’ll use a bit of python magic to pull the data that we want:

PYTHON

native_preproc_T1 = [f for f in preproc_T1 if f not in mni_preproc_T1]
native_preproc_T1

Similarily fMRI data can be pulled by specifying datatype=‘func’ and using the desc argument as appropriate:

Exercise 1

Get the list of all preprocessed functional data
Get the list of functional data in MNI152NLin2009cAsym space
Get the list of functional data in T1w space (native) Note that T1 space fMRI data can be pulled using space=“T1w” (this is unlike the T1w data which required you to do some filtering)

Show me the solution

All the functional data

PYTHON

func_data = layout.get(datatype='func', desc='preproc')

MNI152NLin2009cAsym Functional Data

PYTHON

mni_func_data = layout.get(datatype='func', desc='preproc', space='MNI152NLin2009cAsym')
mni_func_data

Native/T1w space functional data

PYTHON

t1w_func_data = layout.get(datatype='func', desc='preproc', space='T1w')
t1w_func_data

Now that we have a handle on how fMRIPREP preprocessed data is organized and how we can pull this data. Let’s start working with the actual data itself!

Key Points

fMRIPrep stores preprocessed data in a ‘BIDS-like’ fashion
You can pull files using pyBIDS much like how you can navigate raw BIDS data